雌激素受体在人类垂体腺瘤中的表达

Expression of estrogen receptor in human pituitary adenomas

目的检测雌激素受体(estrogenreceptor,ER)在人类不同类型激素垂体腺瘤中的表达,探讨垂体腺瘤中分泌不同类型激素的腺瘤细胞与ER免疫组化阳性细胞之间的关系。方法采用免疫组化S-P法对53例垂体腺瘤标本进行激素分型,检测垂体腺瘤中ER蛋白的表达。采用免疫组化双标法检测多激素分泌型垂体腺瘤中垂体激素合并ER表达的情况。结果53例垂体腺瘤标本中,部分PRL(5/7)、FSH(2/3)、LH(1/1)单激素腺瘤及部分多激素腺瘤有ER蛋白表达,而全部GH、ACTH、TSH单激素腺瘤均无ER蛋白表达,4例无功能腺瘤无ER蛋白表达。在33例多激素型垂体腺瘤标本中,22例有ER蛋白表达,其中PRL+ER双标染色阳性标本10例、LH+ER双标染色阳性标本9例、FSH+ER双标染色阳性标本7例、GH+ER双标染色阳性标本2例,33例标本的ACTH+ER和TSH+ER的双标染色均为阴性。结论垂体腺瘤患者的性别不影响肿瘤组织中ER蛋白的表达。垂体腺瘤中,分泌PRL、LH或FSH的垂体腺瘤细胞可表达ER;分泌ACTH或TSH的垂体腺瘤细胞不表达ER;分泌GH的垂体腺瘤细胞是否表达ER可能与该垂体腺瘤是否同时分泌PRL有关。ER在PRL、LH及FSH垂体腺瘤细胞的发生、发展中发挥作用。

Objective To examine the expression of estrogen receptor（ER） in different hormone-producing pituitary tumors in order to study the relationship between the specific hormone-secreting adenoma cells and the ER-immunoreactive tumorous pituitary cells. Methods Immunohistochemical S-P method was used to identify the hormone-producing adenoma type and to detect the expression of ER protein. Immunohistochemical double-staining method was applied to detect the coexpression of ER and one specific pituitary hormone. Results In 53 adenomas, ER protein was found in some adenomas expressing only PRL （5/7）, FSH（2/3） or LH（1/1）, and in some plurihormonal adenomas, while no ER protein was detected in adenomas expressing only GH, ACTH and TSH. No ER protein was found in 4 nonfunctioning tumors. In 33 plurihormonal pituitary tumors, ER protein was detected in 22 cases. Among them, PRL and ER double staining was found in 10 specimens, LH and ER double staining in 9 specimens, FSH and ER double staining in 7 specimens, and GH and ER double staining in 2 specimens. Double staining did not characterize the nuclear ER positivity in cells containing cytoplasmic reactivity for ACTH or TSH in 33 cases. Conclusion The sex of the patients did not affect the expression of ER in pituitary tumors. The PRL-, LH- or FSH- producing adenoma cells can express ER, while neither ACId- nor TSH- producing adenoma cells express ER. Whether GH-secreting tumorous cells express ER may be related to the pituitary adenoma secreting PRL or not. ER may play an important role in the occurrence and development of the PRL-, LH- or FSH- secreting adenomatous cells.