摘要
本试验研究建立了高效液相色谱法测定鸡蛋中7种磺胺药物(磺胺嘧啶、磺胺二甲基嘧啶、磺胺甲氧哒嗪、磺胺间甲氧嘧啶、磺胺甲恶唑、磺胺间二甲氧嘧啶和磺胺喹恶啉)残留的提取、净化和高效液相色谱分析条件,鸡蛋经二氯甲烷提取、固相萃取、净化处理后上机检测,7种磺胺药物标准曲线的相关系数(R2)为0.9995～0.9999,在0.05～0.2mg/kg浓度范围内,回收率为73.2%～102.6%,变异系数为1.8%～8.7%。检测限为0.01～0.02mg/kg,定量限低于0.05mg/kg。
A high-performance liquid chromatography method for the multi-residues simultaneous determination of sulfadiazine, sulfamethazine, sulfamethoxypyridazine, sulfamonomethoxine, sulfamethoxazole, sulfadimethoxine and sulfaquinoxaline in eggs was described in this paper. The egg samples were extracted with dichloromethane, and cleaned upon by a C18 column solidphase extraction （SPE）. The calibration curves for seven sulfonamides were linear in a concentration range of 0.02- 1.0μg/ml （R^2=0.9995-0.9999）.The recoveries at levels of 0.05,0.1,0.2mg/kg fortified samples ranged from 73.2%-102.6%, with coefficient of variation of 1.8%-8.7%. The limits of detection were 0.02mg/kg and the limits of quantification less than 0.05mg/kg.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第11期183-186,共4页
Food Science
基金
江苏省科技厅攻关项目(BE2003306)
