顶空固相微萃取的分段操作

Study on Multistage Operation of Headspace Solid Phase Micro-extraction of Meat Essence

本文研究了在利用顶空固相微萃取对挥发性物质进行吸附检测时,操作因素对萃取容量的影响。在不同保留时间段,挥发性物质在萃取纤维上的吸附情况受到操作因素的影响不同。在各个时间段,萃取温度都是最大的影响因子。基于以上,提出顶空固相微萃取的分段操作,针对高挥发度的待分析物质采用较低的萃取温度萃取,低挥发度的待分析物质采用较高的萃取温度萃取。

The article studied the quantitative and qualitative impaction of operate-factors on fiber absorption capacity when volatile substance of meat essence was extracted and analysed by applying HS-SPME coupled with GC-MS. The results appeared that in the different Remain Times （RT）, the impactions of each operate-factor on the absorption of volatile substance in the SPME fiber varied. Among them the extracting temperature were the predominant factor. According to the extracting temperature, the multistage operation of HS-SPME was developed, that is the operation should apply relative high extracting temperature for low volatility substance, and apply relative low extracting temperature for high volatility substance.