葛根黄酮提取物对HL-60细胞bcl-2和bax基因表达的影响

Effect of Kudzu Flavonoids Extracts on Expression of bcl-2 and Bax Gene in HL-60 Cell

目的:探讨葛根黄酮提取物对HL-60细胞bcl-2和bax基因表达的影响。方法:用RT-PCR和蛋白质免疫印迹技术检测葛根黄酮提取物、葛根素和大豆甙元处理HL-60后,对bcl-2和bax基因的mRNA和蛋白表达的影响。结果:葛根黄酮提取物、葛根素和大豆甙元处理HL-60细胞后能够上调baxmRNA表达水平,下调bcl-2蛋白表达和上调Bax蛋白表达,且随着处理时间的延长其诱导下调和上调能力增强。结论:葛根黄酮提取物诱导HL-60细胞凋亡与其下调bcl-2mRNA和蛋白表达水平和其上调baxmRNA和蛋白表达水平有关。

Objective： To study the effect of kudzu flavonoids extracts on the expression of bcl-2 and bax gene in HL-60 cells.Methods： To use RT-PCR and western blotting technology to detect the mRNA and protein expression level of bcl-2 and bax gene in the HL-60 cells treated by kudzu flavonoids extracts, pueradn and daidzein. Results： In a time-dependent manner, kudzu flavonoids extracts, puerarin and daidzein increased the bax mRNA and protein expression level and lowered the bcl-2 protein expression level. Conclusion： The ability of kudzu flavonoids extracts could induce HL-60 cells apoptosis in relation to lower the bcl-2 mRNA and protein expression level and increase the bax mRNA and protein expression level.