藤黄灰链霉菌099生产麦拓莱霉素与脂肪酸酯的关系

Relationship between Fatty Acid Ester and Maituolaimycin Production in Streptomyces regensis 099

为确定藤黄灰链霉菌099经聚酮途径合成麦拓莱霉素与初级代谢产物脂肪酸酯间的关系,利用柱层析,对发酵液中的脂肪酸酯加以分离纯化,用核磁共振谱、电喷雾电离质谱鉴定其结构,构建该菌株合理的代谢关系,并通过添加脂肪酸的发酵实验加以验证.结果表明该菌株中不仅存在聚酮合成途径,而且存在脂肪酸酯代谢途径.

To establish the relationship between the biosynthetic pathway of a primary metabolite fatty acid ester and that of a novel macrolide maituolaimycin, a multi-separation chromatographic method was used to separate and purify the fatty acid ester from the fermentation broth of Streptomyces regnesis 099. And the structure of this fatty acid ester was identified using NMR and ESI-MS. A rational model was built in regard to the relationship of the two mentioned above and was validated by the fermentation experiments. The results showed that both polyketide biosynthetic pathway and fatty acid ester biosynthetic pathway existed in the strain, which is helpful to the optimization of fermentation technology.