叶片叶绿素荧光参数用作采后切花菊衰老指标

Assessment of senescence of cut flowers in Chrysanthemum morifolium L.as judged from chlorophyll fluorescence characteristics of leaves on the same shoot.

测定了采后切花菊(Chrysanthemum morifoliumL.)衰老过程中叶片叶绿素含量、叶绿素荧光参数和花朵乙烯释放量的变化.结果显示,不同叶位叶片的衰老进程存在差异,叶片叶绿素含量的下降与荧光参数Fv/Fm、ΦPSⅡ、Fv′/Fm′和qP的变化呈极显著正相关关系.叶片的叶绿素荧光参数Fv′/Fm′、ΦPSⅡ和qP的显著下降先于或同步于叶绿素含量的下降,也早于花朵的乙烯跃变,Fv/Fm的下降迟于叶绿素的降解.认为叶绿素荧光参数Fv′/Fm′、ΦPSⅡ和qP可用作切花菊采后衰老的准确、快速和无损伤预测探针.

Chlorophyll fluorescence parameters were measured by pulse-amplitude modulation （PAM） fluoremetry on the leaves of Chrysanthemum morifolium L. cut flowering shoots, Leaf chlorophyll degradation and flower ethylene release were measured at the same time. Specifically leaf senescence sequence was observed on leaves of different leaf positions. Along with the process of leaf senescence, the correlations between the decrease in chlorophyll content and chlorophyll fluorescence parameters Fv/ Fro,ΦPSⅡ, Fv＇/Fm＇ and qP were remarkable （P 〈0,001）. The significant decrease in parameter Fv＇/ Fro＇, ΦPSⅡ and qP preceded or paralleled the chlorophyll degradation, and appeared before the rise of flower ethylene climacteric, while occurrence of decline in Fv/Fm was later than chlorophyll degradation, The results suggested that chlorophyll fluorescence parameters Fv＇/Fm＇, ΦPSⅡ and qP may provide a suitable, rapid and non-destructlve criterion for estimation of leaf senescence.