BMPR-ⅠA/ⅠB在青少年特发性脊柱侧凸骨髓间质干细胞中的异常表达及意义

Abnormal expression and etiological significance of BMPR-ⅠA/ⅠB in mesenchymal stem cells from adolescent idiopathic scoliosis

目的:从骨髓间质干细胞(MSCs)水平探讨BMPR-ⅠA/ⅠB表达与青少年特发性脊柱侧凸(AIS)发病可能相关的分子机制。方法:30例12—18岁志愿者分为2组:AIS组20例,同年龄正常对照组10例。髂前上棘处穿刺抽取10mL骨髓,肝素抗凝。密度梯度离心法分离MSCs,体外培养并传至P3代行表型鉴定,采用RT-PCR法、Western-blotting法、免疫荧光法检测2组MSCs中BMPR-ⅠA/ⅠB的表达情况。结果:BMPR-ⅠA/ⅠB不论是核酸水平还是蛋白水平的表达,在AIS组均要显著高于正常对照组(P<0.01)。结论:BMPs信号通路中重要的膜表面分子BMPR-ⅠA/ⅠB在MSCs水平表达的异常可能与AIS发病的分子机制相关。

Objective： To investigate a possible related molecular mechanism between pathogenesis of adolescent idiopathic scoliosis and expression of BMPR-IA/IB from mesenchymal stem cells（MSCs） level. Method： 30 volunteers were divided into two groups： AIS group consisted of 20, and the other 10 were normal control group. The human bone marrow anticoagulated by calparine was obtained from anterior superior iliac spine. The MSCs were isolated by density gradient centrifuge from the mononuclear cells, and then the MSCs were cultivated and serial subcultivated in vitro. P3 culture was analyzed by the flow cytometry to detect the surface antigens. The expression of BMPR-IA/IB of MSCs from 2 groups was detected by serial using RT-PCR, Westernblotting and immunofluorescence. Result： The expression of hymenosurface molecule BMPR-IA/IB of MSCs increased obviously in AIS group as compared with control group （P〈0.01）. Conclusion： The abnormal expression of hymenosurface molecule BMPR-IA/IB of MSCs and the important member of BMPs signaling pathway may be related to the molecular mechanism of the pathogenesis of AIS.