慢性充血性心力衰竭患者血浆内源性类洋地黄物质的部分纯化与鉴定

Partial purification and identification of endogenous digitalis-like substance from plasma of patients with chronic congestive heart failure

选用Ｃ１８预处理柱分离出２３例慢性充血性心力衰竭（ＣＨＦ）患者血浆内源性类洋地黄物质（ＥＤＬＳ），应用高压液相色谱（ＨＰＬＣ）对其进行部分纯化，观察部分纯化的ＥＤＬＳ对红细胞摄取８６Ｒｂ的抑制，而对其进行鉴定，比较部分纯化的ＥＤＬＳ与哇巴因在Ｃ１８反相分析柱上的保留时间。结果表明：①ＣＨＦ患者血浆第二份ＨＰＬＣ洗脱物（保留时间３～６分钟）对红细胞摄取８６Ｒｂ具有强烈的抑制作用；②其抑制率为８０％±６％，明显高于其他洗脱物份别及健康对照血浆第二份洗脱物的抑制率（４３％±３％）（均Ｐ＜０．０１）；③部分纯化的ＥＤＬＳ、哇巴因及两者混合物的保留时间基本一致，提示ＥＤＬＳ与哇巴因结构相似。

The endogenous digitalis-like substance(EDLS) was isolated by C18 disposable columns and partially purified by high performance liquid chromotography(HPLC) in the plasma of 23 patients with CHF and 12 healthy controls. Inhibition of partially purified EDLS on 86Rb uptake by intact erythrocytes was observed to determinate the EDLS,The chromatographic retention of partially purified EDLS on the C18 reversed phase column by HPLC was compared with Ouabain.The results showed:①Fraction Ⅱ in the plasma of patients with CHF possessed stronge inhibitive eftect of 86Rb uptake by intact erythrocytes;②The inhibition rate was 80%±8%,which was obviously higher than the other fractions in the same sample and the fraction Ⅱ of normal controls(43%±3%) (all P<0.01).③The retentions of EDLS. Ouabain and the mixture of both were almost equal. It suggests that EDLS may have same structure as Ouabain.