核DNA倍性与骨巨细胞瘤预后的相关性研究

NuclearDNAPloidyandPrognosisofGiantCellTumorofBone

对３１例骨巨细胞瘤标本进行流式细胞计分析，其中Ⅰ级骨巨细胞瘤９例，Ⅱ级１７例，Ⅲ级５例，二倍体８例，近二倍体３例，异倍体２０例。Ｓ期细胞百分数Ⅰ级为１５．８％±３．４％，Ⅱ级为２１．５％±６．２％，Ⅲ级为２５．９％±４．２％，采用多元逐步回归的统计学方法分析１１种因素在骨巨细胞瘤预后中的意义，结果显示骨巨细胞瘤的预后与手术方式呈负相关，与核ＤＮＡ呈正相关，与其它因素关系甚微，并且在前二者中，以手术方式的作用更为重要。此外，对２例复发前后的骨巨细胞瘤标本进行了对比研究，发现除病理学分级无改变外。

FlowcytometricDNAanalysiswasperformedon31casesofgiantceltumorofbone(GCT),therewere9casesofⅠgrade,17Ⅱgrade,5Ⅲgradeinthisgroup,and8casesofdiploidytu-mor,3near-diploidy,20aneuploidy.TheS-phasecelpercentagewas15.8%±3.4%inⅠgradeGCT,21.5%±6.2%inⅡgrade,25.9%±4.2%inⅢgrade.Wedealt11prognosticfactorsinflu-encingonGCT(includingnuclearDNAploidyandS-phasecelpercentage)withmultipleprogressivestatisticmethod,theresultsshowedtherewerenegativecorrelationbewteenprognosisandoperativemethod,positivecorrelationwithDNAploidy,littlerelationshipwithotherfactors,andtheoperativemethodplayedmoreimportantroleontheprognosis.WedidcomparativestudyofFlowCytometryon2pre-andpost-recurrencesamples,foundthatnuclearDNAcontent,S-phasepercentageincreasedinpostrecurenceunlessnochangeofpathologicalgrade.