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海南黎族人群细胞色素P4502C19基因多态性的研究 被引量：9

Genetic polymorphism of cytochrome P450 2C19 in Li population of Hainan Island.
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摘要 目的研究细胞色素P4502C19(CYP2C19)在海南黎族人群中的遗传多态性征。方法应用限制性片段长度多态性分析法(PCR-RFLP)对165名黎族健康志愿者进行了CYP2C19基因多态性分析。结果wt、m1和m2三种等位基因在海南黎族健康人群中的发生频率分别为61%、36%和3%。其中wt/wt基因型的发生率为38%(62/165),m1/wt和m2/wt基因型的发生率分别为42%(70/165)和5%(8/165),m1/m1和m1/m2基因型的发生率分别为14%(23/165)和1%(2/165),未发现m2/m2基因型个体。结论海南黎族人群的CYP2C19的基因多态性与其它民族相比具有一定的差异性。 Objective To assess the genotype pattern distribution of cytochrome P450 2C19 in Li ethnic population of Hainan Island. Methods One hundred and sixty - five unrelated, healthy Li Nationality subjects, including 79 males and 86 females, aged froml8 to 25, were genotyped for CYP2C19 by polymerase chain reaction （PCR） and restricted fragment length polymorphism （RFLP）. Results The frequency of wt, m1 and m2 alleles were 0.61, 0.36 and 0.03, respectively. 62 subjects were homozy- gous for wild- type （wt/wt）, 23 subjects were homozygous of m1 （m1/m1）, and 80 subjects were heterozygous of m1 or m2 （wt/ m1, wt/m2 or m1/m2）, no homozygous genotype for m2 （m2/m2）. Conclusion The genotyping of CYP2C19 in Li Nationality population of Hainan island is diverse compared with other nationality population.
作者 付琼瑶 李佩琼 王九辉 蔡望伟
机构地区 海南医学院
出处 《中国热带医学》 CAS 2005年第9期1807-1808,共2页 China Tropical Medicine
基金 海南省教育厅科研基金资助(HjKj200424)
关键词 黎族 CYP2C19 基因多态性 Li Nationality CYP2C19 Genetic polymorphism
分类号 R333.6 [医药卫生—人体生理学]
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参考文献10

  • 1Wilkinson GR,Guengerich FP,Branch RA.Genetic polymorphism of S-mephenytoin 4'-hydroxylation[J].Pharmacol Ther 1989,43:53～78.
  • 2De Morais SMF,Wilkinson GR,Blaisdell J,et al.The majoR genetic defect responsible for the polymorphism of S-mephenytoin in humans[J].J Bio Chem,1994,269,15419～15422.
  • 3De Morais SMF,Wilkinson GR,Blaisdell J,et al.Identification a new genetic defect responsible for the polymorphism of S-mephenytoin in Japanese[J].Mol Pharmacol,1994,46,594～598.
  • 4De Morais SM,Goldstein JA,Xie HG,et al.Genetic analysis of the s-mephenytoin polymorphism in a Chinese population[J].Clin Pharmacol Ther,1995,58:404～411.
  • 5Lahiri DK,Schuabel B.DNA isolation by a rapid method from human blood samples:effects of MgCl2,EDTA,storage time,and temperature on DNA yield and quality[J].Bio chem Genet,1993,32∶321～328.
  • 6He N,Yan FX,Huang SL,Wang W,Xiao ZS,Liu ZQ,Zhou HH.CYP2C19 genotype and S-mephenytoin 4'-hydroxylation phenotype in a Chinese Dai population[J].Eur J Clin Pharmacol,2002,Apr;58(1):15～18.
  • 7Xiao ZS,Goldstein JA,Xie HG,,et al.Differences in the incidence of the CYP2C19 polymorphism affecting the S-mephenytoin phenotype in Chinese Han and Bai populations and identification of a new rare CYP2C19 mutant allele[J].J Pharmacol Exp Ther,1997,281:604～609.
  • 8牛春燕,关丽,罗金燕.维吾尔族健康人群CYP2C19基因多态性分析[J].新疆医科大学学报,2005,28(3):247-249. 被引量：8
  • 9Brockmoller J,Cascorbi I,Kerb R,Roots I.Combined analysis of inherited polymorphisms in arylamine N-acetyltransferase 2,glutathione S-transferases M1 and T1,microsomal epoxide hydrolase,and cytochrome P450 enzymes as modulators of bladder cancer risk[J].Cancer Res,1996,56(17):3915～3925.
  • 10Tsuneoka Y,Fukushima K,Matsuo Y,Ichikawa Y,Watanabe Y.Genotype analysis of the CYP2C19 gene in the Japanese population[J].Life Sci,1996,59(20):1711～1715.

二级参考文献7

  • 1Goldstein JA, Faletto MB, Romkes M, et al. Evidence that CYPZC19 is the major (S)-mephenytoin 4'-hydroxylase in man[J]. Biochemistry, 1994,33: 1743-1752.
  • 2de Morais SMF, Wilkinson GR, Blaisdell J, et al. The major genetic defect responsible /or the polymorphism of S- mephenytoin in humans[J]. J Biol Chem, 1994,269:15419-15422.
  • 3de Morais SMF, Wilkinson GR, Blaisdell J, et al. Identification of a new genetic defect responsible for the polymorphism of (S)- mephenytoin metabolism in Japanese[J]. Mol Pharmacol, 1994,46 :594-598.
  • 4Weide JVD, Steljan LSW. Review article: Cytochrom P450 enzyme: genetic polymorphisms and impact on clinical pharmacology[J]. Ann Clin Biochem, 1999, 36:722-729.
  • 5Wedlund PJ, Aslanian WS, McAllister CB, et al. Frenquency of mephenytoin hydroxylation deficiency in a Caucasian population: Idependence from debrisoquine hydroxylation deficiency[J]. Clin Pharmacol Ther, 1984, 36(6):773.
  • 6Wrighton SA, Stevens JC, Beeker GW, et al. Isolation and characterization of human liver eytoehrome P4502C19: correlation between 2C19 and S-mephenytoin 4′- hydroxylation[J].Arch of Biochem and Biophys, 1993,306(1):240.
  • 7Kubota T, Chiba K, Ishizaki T. Genotyping of S-mephenytoin 4′-hydroxylation in an extend Japanese population [J]. Clin Pharmacol Ther, 1996,60(2) :661-666.

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中国热带医学
2005年 第9期

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