摘要
目的观察缺氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)在大鼠缺血再灌注脑组织中的表达,探讨其表达的意义。方法SD大鼠随机分为正常组、单纯缺血组、缺血再灌注组,应用免疫组织化学方法检测HIF-1α在大鼠大脑中动脉缺血区的表达。结果正常组未见HIF-1α表达,缺血再灌注组HIF-1α的表达显著高于单纯缺血组(P<0·05)。结论局灶性脑缺血可诱导HIF-1α的表达且HIF-1α在发生缺血再灌注后表达增加。
Objective To investigate the expression of HIF- 1α after ischemia - reperfusion of brain in rats. Methods 36 rats were divided into 3 groups and copied the model of ischemia - reperfusion of brain in rats for investigating the expression and significance of HIF- 1α by using SABC immunohistochemistry. Results In the normal group, positive expression of HIF - 1α was 0%, while 41.7% in ischemia group and 91.7% in isehemia - reperfusion group. The positive expression rate of HIF- 1α was significantly higher in ischemia - reperfusion group （P 〈 0.05）. Conclusion HIF- 1α was over expressed in brain tissues after is- ehemia - reperfusion.
出处
《中国热带医学》
CAS
2005年第9期1809-1810,共2页
China Tropical Medicine
