氨利酮诱发V_(79)细胞HGPRT位点突变研究

THE INDUCTION OF 6-THIOGUANINE-RESISTANT MUTANTSV79 CHINESE HAMSTER CELLS BY AMRINONE

本文观察了国产抗心血管疾病新药氨利酮在V_(79)细胞HGPRT位点（hgpoxanthine-guanine phos-phoribosyl transferase locus，次黄嘌呤鸟嘌呤转磷酸核糖基酸位点）正向突变试验系统中的诱变作用。结果表明：氟利酮在-S9情况下，各浓度组突变体频率（mutant frequency、MF）与对照组相比均未达到MF≥3SMF的标准，也无剂量反应关系；但相同浓度在+S9情况下，除高浓度组（200μg/ml）外，其余三组MF随浓度增加而升高，且次高浓度组（100μg/ml）MF是对照组的3.8倍，故在本试验条件下氨利硐是需活化的诱变剂。

Inducibility of 6-thioguanine-resistant mutants by a new medicine-amrinone was investigated. In the absence of S9. no mutagenic effect was found. In the presence of S9, mutant frequencies were increased with am-rinone concentration from 10uM to 100uM, but decreased at 200uM. At 100uM mutant frequency was increased more than three-fold over spontaneous mutant values. Thus it was suggested that amrinone can induce mammalian cells gene mutation.