纳米Ag_2O_2-PbO_2化学修饰电极对大肠杆菌细胞膜壁和DNA损伤的研究

Studies on Nano Ag_2O_2-PbO_2 Modified Electrode-induced Cell Membranes and DNA Damage of E.coli

制备了一种新型纳米Ag2O2-PbO2修饰电极,在选定的正电位下,电极表面产生羟基自由基(.OH).通过测定产生的脂质过氧化物和漏出蛋白质的量来研究羟基自由基对大肠杆菌细胞膜壁损伤的情况,并运用电泳和DNA测序的方法对大肠杆菌质粒DNA的损伤及其对序列变化进行了研究.

The fabrication of a novel nano Ag2O2-PbO2 modified electrode is described. Hydroxide radical could be generated on the surface of the electrode at an optimized potential. Lipid peroxidate and transudatory protein were detected to show destruction of cell membrane of E. coli treated by Ag2O2-PbO2 modified electrode. DNA damage of E. coli was studied in detail with electrophoresis and DNA sequence determination. The results show that cracking of plasmid DNA and mutation of gene sequence could be induced by nano Ag2O2-PbO2 modified electrode.