骨肽注射液对去卵巢大鼠骨组织中TGF-β1及Fas的影响

The Effect of Ossotide injection on TGF-β1 and Fas in Bone in Ovariectomized Rats.

目的:观察骨肽注射液对去卵巢大鼠骨组织中转化生长因子-β(TGF-β1)及F as的影响。方法:复制骨质疏松症大鼠模型,假手术组仅摘除小段肠系膜。各组动物术后,以骨肽为治疗药物,苯甲酸雌二醇注射液为阳性对照,生理盐水为阴性对照,分别给药3个月后处死取材。应用免疫组化sABC法检测骨组织中TGF-β1和F as抗原的表达情况。骨肽高、低剂量组骨组织中TGF-β1及F as抗原表达的阳性颗粒的平均灰度、阳性单位值较模型组明显增高(P<0.05);而与假手术组比较则无明显差异(P>0.05)。结论:骨肽注射液能促进成骨细胞形成活性、抑制破骨细胞吸收活性,其作用机理之一可能是通过调控某些影响骨形成及骨吸收的局部细胞生长因子及凋亡基因而达到的。

Objective：To investigate the effects of Ossotide injection on TGF-β1 and Fas in bone in ovariectomized Rats. Methods：Ovariectomy was used in all of model, Ossotide and Estrogen. The small segment mesentery was cut in Sham. All animal were fed in nomal ways for three months after operation. Rats of Ossotide were given Ossotide injection, rats of Estrogen were given nilestriol as positive control, and rats of Model were given Saline as negative control for three months respectively. The methods of immunohistochemistry sABC were used to detect the expressions of Transforming growth factor-β1（TGF-β1 ） and Fas antigen in bone. Results：TGF-β1 was expressed chiefly in osteoblastic membrane and plasm but Fas antigen was expressed chiefly in osteoclastic plasm in bone. Average gray scale and positive unit of Ossotide by expressions of TGF-β1 in osteoblast and Fas in osteoclast singnificantly increased more than that of model（P〈 0. 05） and these data of Ossotide have no difference compared with Sham （P〈0. 05）. Conclusions：Ossotide injection can promate the activity of bone formation of osteoblast and depress the activity of bone absorption of osteoclast. One of mechanism of Ossotide injection on Osteoporosis is induced by＇ ovariectomy in rats is the regulation of some local cell growth factor and apoptosis gene affecting bone formation and bone absorption.