p53、rasp21及C-myc癌基因蛋白在胃癌中的表达及分布特征

Expression and distribution feature of p53, Rasp21 and C-myc oncoproteins in carcinoma of stomach

目的通过对胃癌标本中p53、rasp21及Cmyc癌基因蛋白的表达分析,探讨其在胃癌发生中的作用。方法用免疫组化方法检测了60例胃癌标本中p53、rasp21及Cmyc的表达及分布。结果p53、rasp21及Cmyc在胃癌中的阳性表达分别为51.7%、40.0%、66.7%;胃癌旁正常黏膜上皮的阳性率分别为1.6%、13.3%、15.0%,与肿瘤区相比较有显著差异(P<0.01)。rasp21及Cmyc在肿瘤区与肿瘤移行区、癌旁正常黏膜区染色强度比较有差异(P<0.05)。p53及Cmyc阳性表达与肿瘤的分化程度有关,即分化越差,其阳性表达越高。结论p53、rasp21及Cmyc与胃癌的发生、发展密切相关,p53及Cmyc在肿瘤分化过程中可能起重要作用。

Objective To investigate the role of p53, rasp21 and C-myc in gastric carcinogenesis. Methods Expression and distribution feature of p53, rasp21 and C-myc in 60 cases of human gastric cancer were detected with irnmunohistochemical SP （Streptavidin-Peroxidase） staining. Results The positive rate of expression was respectively 51.7% for p53, 40. 0% for rasp21, and 66. 7% for C-myc, while in the gastric mucous membrane adjacent to carcinoma they were respectively 1.6% for p53, 13.3% for rasp21, and 15.0% for C-myc. The differences in expression of p53, rasp21 and C-myc oncoproteins in carcinoma and non-carcinoma tissues were very significant （P〈0. 01）, and there was significant difference （P%0.05） in the density of rasp21 and C-myc staining between tumor region, translated region, and adjacent mucous membrane. The positive expression of p53 and C-myc was related to the differentiation of tumor tissues, the poorer the differentiation, the higher the positive expression. Conclusion p53, rasp21 and C-myc were closely correlated with pathogenesis and development of gastric carcinoma, and the expression of p53 and C-myc may contribute to the degree of tumor differentiation.