牙本质粘结剂对人牙髓细胞毒性的体外研究

Cytotoxicity of dental bonding agents to human dental pulp cells in vitro

目的:评价全酸蚀牙本质粘结剂和自酸蚀牙本质粘结剂对人牙髓细胞的毒性作用。方法:用人牙髓细胞为实验细胞,采用MTT比色分析法,对4种牙本质粘结剂(Prime&Bond NT,Single Bond,XenoⅢ,iBond)进行体外细胞毒性研究。结果:不同浓度的粘结剂稀释液均可使人牙髓细胞的形态有所改变。4种牙本质粘结剂的细胞毒性有显著性差异,且作用时间和浓度的改变对其细胞毒性有影响。全酸蚀粘结剂比自酸蚀粘结剂的细胞毒性强。结论:4种牙本质粘结剂在体外对人牙髓细胞均有一定程度的细胞毒性,其中Single Bond的毒性较强,临床使用粘结剂时应合理选择粘结剂和掌握固化时间。

Objective：To evaluate the cytotoxicity of different dental bonding agents to human dental pulp cells. Methods：Four dental bonding agents（ Prime ＆ Bond NT,Single Bond,Xeno Ⅲ and iBond） were diluted with the culture medium by a ratio of 1： 1 000,1：2 000 and 1：4 000（v/v） , and the fourth passage of dental pulp cells were then exposed to agent dilutions for 12, 24 and 48 h respectively, then the morphological changes of pulp cells were observed with microscopy and cytotoxicity was analyzed with modified 3-（4,5-dimethyl-thiazol-2-yl） -2,5-diphenyl-tetrazolium bromide （MTT） assay. Results ：There was significant influence of the type of dental bonding agents, exposure time and concentration on the cell morphology. Total-etch system was more toxic than self-etch system. Conclusion：Dental bonding agents are toxic to pulp cells,Single Bond is the strongest cytotoxic agent among the 4 adhesive agents.