禽流感病毒夹心ELISA诊断试剂盒的研制及应用

Development and Application of Sandwich Enzyme-linked Immunosorbent Assay Test Kit for Detecting Avian Influenza Virus Antigen

将成熟的禽流感病毒(AIV)夹心ELISA快速检测方法组装成诊断试剂盒.试剂盒由1～11号试剂、一块酶标板和一份说明书组成.对试剂盒内各组份进行了特异性、敏感性、重复性及保存期的测定.结果表明,在-10℃下能保存6个月,而且特异性强、敏感性高、重复性好,操作简单、方便,能快速、准确地检测出样品中是否带有AIV抗原.先后制备了8个批次的诊断试剂盒,于湖北、安徽及河南等地的养禽场、兽医站、农科院、农业院校等化验室应用,取得了良好的效果.

A Sandwich enzyme-linked immunosobent assay test kit based on previous work was developed for detection of avian influenza virus （AIV） antigen. The kit consists of eleven reagents and a piece of 16-well microtiter plate. There was highly specificity, sensitivity and repeatability indetecting AIV antigen with the test kit. It was stable well when storing at -10℃ for six months. It is easy to manipulate and the result can be reported within 3 hours. So far. the kit has been successfully used in some provinces such as Hubei. Anhui and Henan and so on. The test kit was proved to be fit for all related fields detecting AIV antigen.