番鸭源新城疫病毒F蛋白基因的克隆及序列分析

Cloning and Sequence Analysis of F Protein Gene of Paramyxovirus Type Ⅰ from Muscovy Duck

利用RT-PCR技术对番鸭源新城疫病毒FP1/02株的F蛋白基因进行分段扩增、定向克隆到pMD 18-T SimpleVector质粒载体,然后测定其核苷酸序列,拼接出F基因全序列,并推导出其相应的氨基酸序列.FP1/02株的F蛋白基因全长1690 bp,编码553个氨基酸,其裂解位点的氨基酸序列为112R-R-Q-K-R-F117,具有强毒株特有的氨基酸序列结构特征.核苷酸序列分析结果表明,FP1/02株与其他不同源新城疫病毒毒株之间的核苷酸序列同源性为87.2%～93.3%.

Full-length F protein gene of the FP1/02 strain of Newcastle disease virus（NDV） isolated from muscovy duck was amplified by using reverse transcription polymerase chain reaction（RT-PCR） and cloned into pMD 18-T simple vector. The sequence of F protein gene of FP1/02 strain was determined with its full-length of 1690 bp coding 553 amino acid（AA）. The amino acid sequence in the cleavage site of the deduced peptide is ^112R-R-Q-K-R-F^112,which exists only in a virulent NDV strain. The result of sequence analysis of F protein gene in full-length indicated that the homology between FP1/02 isolate and other NDV strains would be from 87.2% to 93. 3%.