牛卵泡颗粒细胞无血清培养的形态学观察

Morphological Characteristics of Proliferation and Differentiation of Bovine Granulosa Cells in Serum-free Culture System

以M cCoy′s5a为基础培养液添加硒、转铁蛋白、雄烯二酮、谷氨酰胺,对牛卵巢上大(直径>8 mm)、中(4 mm<直径<8 mm)、小(直径<4 mm)非闭锁卵泡的颗粒细胞进行无血清培养。结果表明,M cCoy′s5a无血清培养中的颗粒细胞形态与分化特点类似于体内情况,说明颗粒细胞在该培养条件下,其功能可能更接近体内的生理状况。

A new culture in vitro method for bovine granulosa cells from non -atretic follicles at different size（small：〈4 mm,medium：4-8 mm,and largt： 〉8 mm diameter） and a serum-frue culture system using McCoy＇s 5a medium（pH7.4） supple-mented with 20 mmol/L Hepes,3 mmol/L L-glutamine.0.1% BSA .29 μg/L, testosterone.2. 5 mg/L transterrin and 4μg/L setenium were developed, The results indicated that morphological characteristics of granulosn cells was similar to that of in vivo in this long-term culture system.