慢性束缚应激大鼠中枢糖皮质激素受体变化及中药复方的影响

EFFECTS OF CHINESE HERBS ON GLUCOCORTICOID RECEPTOR IN RAT BRAIN REGIONS WITH CHRONIC IMMOBILIZATION STRESS

目的:研究慢性束缚应激时大鼠脑内糖皮质激素受体的变化以及逍遥散、四君子汤、金匮肾气丸三种中药复方对其影响。方法:制作大鼠束缚应激模型,用特制束缚架连续束缚7 d与21 d,每天3 h用免疫组织化学方法结合图像分析检测中枢(海马CA1区、齿状回、大脑皮质)糖皮质激素受体的变化。结果:慢性束缚应激后,大鼠海马CA1区、大脑皮层和齿状回GR免疫反应阳性细胞平均总面积和阳性细胞数目在慢性应激的早期(7d模型组)明显增多(P<0.05),免疫反应强度明显增强(P<0.01)。在慢性应激的后期(21 d模型组),则表现为相关脑区GR免疫反应阳性细胞平均总面积和阳性细胞数目均明显减少(P<0.05),免疫反应强度明显减弱(P<0.01)。中药复方各组相关脑区神经元GR免疫反应阳性细胞平均总面积和阳性细胞数目较21 d模型组明显增高,免疫反应强度明显增强,三给药组之间并无明显差异,说明三给药组均能使GR含量保持于较高的状态,同时能保持GR免疫活性,其中又以逍遥散作用为明显。结论:逍遥散、四君子汤和金匮肾气丸明显逆转糖皮质激素受体下降趋势。

Aim： To investigate the changes of glucocorticoid receptor（GR） in rat brain regions with chronic immobilization stress and the influence of Chinese herbs. Methods： We copied the rat model of chronic immobilization stress（ 180 min daily, repeated 7 days or 21 days）, and characterized the changes of GR in hippocampus CA1 , cortex, dentate gyrus via immunohistochemistry integrated image analysis. Results： The contents of glucocorticoid receptors in hippocampus CA1, cortex, dentate gyrus were increased and the intensi- ty of immunity reaction was significantly stronger in the model group of 7 days than that in the normal control group（P〈0.05）. The contents of GR were significantly decreased and the intensity of immunity reaction was weaken in the model group of 21 days（P 〈 0.05）. Conclusion： Compared with the model group of 21 days, the contents of GR were significantly increased and immunity reaction was intensified in three groups of treatment with chinese herbs（P〈0.05） , in which the group treated with soothing-liver herbs Xiaoyaopowder was the best.