面神经不同程度损伤后转化生长因子β的表达

Expression of transforming growth factor beta after facial nerve injury of different degree

目的:观察兔不同程度面神经损伤后,损伤局部与创伤愈合和神经再生相关的转化生长因子β1表达的变化。方法:实验于2004-06-09/2005-02-30在解放军第四军医大学西京医院实验外科完成。取成年长耳白毛家兔68只,随机分为6组,对照组(n=9) 和5个损伤组,损伤组分别在双侧面神经颊面干造成暴露(n=13)、牵拉 (n=10)、压榨(n=11)、挤压(n=12)及切断(n=13)5种损伤,于伤后1 h、 1,3,7 d分别取材。苏木精-伊红染色行组织学观察;转化生长因子β1 免疫组化染色,按照细胞着色的程度行染色值半定量分析。结果:68只兔进入结果分析。对照组转化生长因子β1染色值为1．27±0.31, 暴露组、牵拉组各时间点转化生长因子β1表达无明显变化,压榨组、挤压组、切断组面神经损伤后1 h和1 d转化生长因子β1表达升高(P<0．05), 3 d时下降,7 d时再度出现升高,显著高于对照组(1．99±0．32,2．04±0．29． 2．56±0．42,P<0．05)。各组间相比可见,同一时间点不同损伤组的染色值为切断组>挤压组>压榨组>牵拉组>暴露组。结论:①不同程度面神经损伤后,损伤局部的转化生长因子β1水平随损伤程度加重而增高。②损伤后转化生长因子β1水平在1 d和7 d各出现1个高峰。提示转化生长因子β1的表达在面神经损伤后的病理变化与修复再生过程中具有重要意义。

AIM： To observe the change of local transforming growth factor beta （TGF-β1） expression, which is correlated with wound healing and neural regeneration in rabbits after facial nerve injury of different degree. METHODS： The experiment was carried out in the laboratory surgery department of Xijing Hospital, the Fourth Military Medical University of Chinese PLA between June 2004 and February 2005. Sixty-eight adult rabbits were randomly divided into 6 groups： control group （n=9） and 5 injury groups, rats in the injury groups were made into bilateral facial nerve injury models of exposure （n=13）, stretch （n=10）, compression （n=11）, crush （n=12） and cut （n=13）. The materials were taken at 1 hour and 1, 3 and 7 days after injury. The hematoxylin-eosin （HE） staining was applied in the pathological observation. The TGF-β1 was detected with immunohistochemical staining, and the semi-quantitative analysis of the stained value was performed according to the degree of cell pigmenting. RESULTS： All the 68 rabbits were involved in the analysis of results. The stained value of TGF-β1in the control group was 1.27±0.31, the expression of TGF-β1 in the exposure group and stretch group had no obvious changes at different time point. In the compression group, crush group and cut group, the expressions of TGF-β1 were increased at 1 hour and 1 day after the facial nerve injury （P〈 0.05）, decreased at 3 days, and increased again at 7 days, which were all significantly higher than that in the control group （1.99±0.32, 2.04±0.29, 2.56±0.42, P〈 0.05）. At the same time point, the stained value was that cut group〉 crush group〉 compression group〉 c ompression group〉 exposure group. CONCLUSION： ①After facial nerve injury of different degree, the local TGF-β1level is increased with the aggravation of injured severity. ② The TGF-β1 level had a peak at 1 and 7 days after injury respectively, indicating that TGF-β1 expression plays an important role in the pathological changes and recovery after facial nerve injury.