鼻咽癌患者染色体断裂热点与SCE高发位点、脆性部位、原癌基因位点的相关研究

CORRELATION OF BREAK HOT SPOTS FROM PATIENTS WITH NASOPHARYNGEAL CARCINOMA AND HIGH-FREQUENCY SCE POINTS,FRAGILE SITES,PROTO-ONCOGENE LOCI

采用在染色体标本上同时显示ＳＣＥ及Ｇ带带型的方法，对鼻咽癌患者染色体断裂热点与ＳＣＥ高发位点、染色体脆性部位及原癌基因位点之间的相关性进行了分析。结果表明：患者的ＳＣＥ频率、染色体畸变率均显著的高于对照组（Ｐ＜０．０１），患者的染色体断裂点和ＳＣＥ位点都主要分布在染色体的Ａ、Ｂ、Ｃ、Ｄ组和浅带上，且两者所累及的染色体号存在着明显的相关（ｒ＝０．９５７６，Ｐ＜０．０１），患者的１５个断裂热点与ＳＣＥ高发位点的一致率为５３．３３％，与脆性部位的一致率为８０％，与原癌基因位点的一致率为４０％

Using a technique for simultaneous demonstration of G bands and SCE,we have analyzed the correlation of the break hot spots and high-frequency SCE points,fragile sites,proto-oncogenes loci in cultured peripheral blood lymphocytes from 20 untreated patients with nasopharyngeal carcinoma.The results show that chromosome aberration rates and SCE frequencies in patients are significantly higher than that in normal individuals(P<0.01).The breakpoints and SCE points were correlated(r=0.9576,P<0.01),and mainly distributed in A,B,C,D groups and inter-bands region.The break hot spots involved in patients presented a coincidence with the location of fragile sites(80%),the high-frequency SCE points(53.33%) and the proto-oncogene loci(40%).