DAB诱发大白鼠肝癌过程中细胞质膜糖蛋白变化的研究

STUDY ON CHANGES OF GLYCOPROTEINS IN PLASMA MEMBRANE FROM RAT LIVERS DURINGHEPATOCARCINOGENESIS INDUCED BY DAB

以二甲基氨基偶氮苯（ＤＡＢ）诱发大白鼠肝癌为模型，观察了在诱癌过程中和肝癌形成后，肝细胞质膜上糖蛋白的变化。用不连续蔗糖密度梯度离心法制备肝细胞质膜，用ＣｏｎＡ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析法分离出肝细胞质膜糖蛋白，再进行ＳＤＳ－ＰＡＧＥ电泳分析。结果表明诱癌４ｗｋ，ｌ６ｗｋ的肝细胞质膜搪蛋白成份和正常肝比较无显著差异。肝癌细胞质膜上糖蛋白的ＳＤＳ－ＰＡＧＥ电泳图谱和正常肝比较，一些高分子量的糖蛋白缺失，而低分子量的糖蛋白含量增加．并显示了一条分子量为６．６１ＫＤ的新的糖蛋白带。

We had observed the changes of glycoproteins in plasma membranes of rat Hepa-tocyte during hepatocarcinogenesis induced by DAB. The plasma membranes of liver cellwere taken by discontinuous sucrose density gradient centrifugation inethod. The SDS-PAGE patterns of glycoproteins of plasma membranes prepared from Con A affinity chro-matography show no difference between normal liver and livers of 4 weeks and l6 weeks af-ter DAB administration. But in the SDS-PAGE pattern of hepatoma,certain high molecularweight protein bands disappeared. On the contrary, the contents of lower molecular weightprotein increased and a band of new proteiri with molecular weight 6.61 KD was shown.