转染了细胞色素P450　1A1基因重组表达质粒的CHL细胞（CHL一1A1）可代谢活化多环芳烃类化合物

BIOACTIVATION OF POLYCYCLIC ACROMATICHYDROCARBONS IN CHL-1A1 CELL LINE ESTABLISHED BYTRANSFECTION OF RECOMBINANT HUMAN CYT P45O 1A1cDNA EXPRESSION PLASMID

本研究应用我室建立的稳定表达ＣｙｔＰ４５０１Ａ１的转基因细胞株ＣＨＬ－１Ａ１对一组前致突变物／前致癌物进行双核细胞微核试验,同时以母细胞系ＣＨＬ为对照,结果表明,在不加Ｓ９的情况下,五种前致突变物,苯并(α)芘、３－甲基胆蒽、苯并蒽、二甲基苯蒽和蒽均能诱导ＣＨＬ－１Ａ１细胞的微核率显著增高,并具有剂量反应关系,证明ＣＨＬ－１Ａ１细胞具备代谢活化多环芳烃类化合物的能力。因此,转基因细胞株ＣＨＬ－１Ａ１在化学致癌物的遗传毒理学研究方面具有重要的应用价值。

The bioactivation of a panel of promutagens[Benzo(a) pyrene(B(a)P),3-Methylcholanthrene(3-MC),1,2-Benzanthrance(BA),7, 12-Dimethylbenz(a)an-thracene(DMBA),Anthracene(A)] in CHL cell line and that transfected with recombinanthuman Cyt P450 1A1 cDNA expression plasmid(CHL-1Al cell line)were studied.The endpoint selected was the induction of micronuclei assay. All chemicals tested induced repro-ducible micronuclei,and the dose-response relations were observed. This study demon-strates the benefit of using transgenic cell line CHL-1A1 in short term mutagenicity assayin vitro for polycyclic aromatic hydrocarbons and also the potential use in metabolic pathwaystudy.