云南彝族2型糖尿病与HLA-DQA1等位基因多态性的关联研究

Association between the polymorphism of HLA-DQA1 alleles and type 2 diabetes in Yi nationality of Yunnan

目的探讨云南彝族2型糖尿病与HLA-DQA1等位基因多态性的关联性。方法采用聚合酶链反应-序列特异性引物技术,对58例云南楚雄地区彝族2型糖尿病患者和同地区82名彝族正常对照者进行基因分型,做2型糖尿病与HLA-DQA1等位基因多态性的关联分析。结果云南彝族2型糖尿病组与彝族对照组比较,HLA-DQA10301等位基因频率明显高于对照组(P=0.002,RR=3.097);HLA-DQA10601等位基因频率明显低于对照组(P=0.025,RR=0.429),差异有统计学意义。结论HLA-DQA10301是云南彝族2型糖尿病的易感基因;HLA-DQA10601是云南彝族2型糖尿病的保护基因。

Objective To investigate the association between the polymorphism of HLA-DQA1 alleles and type 2 diabetes in Yi nationality of Yunnan. Methods Polymerase chain reaction with sequence-specific primers （PCR-SSP） genotyping method was conducted in 58 ethnic Yi patients with type 2 diabetes mellitus and 82 ethnically matched controls from Chuxiong of Yunnan. Then a study was made on the association between the polymorphism of HLA-DQA1 alleles and type 2 diabetes mellitus. Results The frenquency of HLA-DQA1 ＊ 0301 allele in the patients with type 2 diabetes mellitus was significantly higher than that in the heathy controls （ P = 0.002, RR = 3.097）, and the frenquency of HLA- DQA1 ＊ 0601 in the patients was significantly lower （ P = 0.025, RR = 0. 429）. Conclusion In Yi nationality of Yunnan, HLA-DQA1 ＊ 0301 allele may be a susceptible gene and the HLA-DQA1 ＊ 0601 allele may protect individuals from the risk of diabetes mellitus.