广西融水县苗族六个短串联重复序列基因座的研究

Genetic polymorphism of 6 short-tandem repeat loci in Miao minority group of Rongshui county in Guangxi province

目的了解广西融水县苗族人群无关个体的6个短串联重复序列基因座D7S820,D13S317,D16S539,HUMCSF1PO,HUMTPOX,HUMTH01遗传多态性分布情况,获得相应多态位点的群体遗传学数据。方法用枸橼酸钠抗凝法采集血样,酚-氯仿抽提法提取DNA,应用AmplFSTRIdentifilerTM荧光标记复合扩增技术和ABI Prism○R3100型遗传分析仪进行检测分析。结果6个基因座D7S820,D13S317,D16S539,HUMCSF1PO,HUMTPOX,HUMTH01分别检测出7、8、6、7、5、7个等位基因,基因型的分布符合Hardy-Weinberg平衡定律(P>0.05),其累积个人识别力0.999995,累积非父排除率0.9959,多态信息总量0.9987。结论上述6个基因座具有高度多态性和遗传稳定性,符合孟德尔遗传规律,可为广西苗族的民族遗传学研究和法医学鉴定的提供基础数据。

Objective To investigate the distributions of six short-tandem repeat （STR） loci, namely D7S820, D13S317, D16S539,HUMCSF1PO,HUMTPOX and HUMTH01, in Miao minority group at Rongshui county in Guangxi province and construct the relevant genetic database. Methods Sodium-citrated blood specimens were collected from 208 healthy unrelated Miao individuals in Rongshui county. The DNAs from the specimens were extracted with phenolchloroform method; AmpFSTR Identifiler^TM PCR Amplification Kit was used to amplify the extracted DNAs, and 3100 Genetic Analyzer was used to analyze and screen the amplified products. Results In this study, 7,8,6,7,5,7 alleles were observed at the 6 STR loci respectively. The expected distribution of genotype accorded with Hardy-Weinbery equilibriam. The total discrimination power, cumulative paternity exclusion power and total polymorphism information were 0. 999995, 0. 9959 and 0. 9987 respectively. Conclusion The results demonstrate that these 6 STR loci are of high polymorphism and hereditary stability and are in accord with Mendel＇s law. The data obtained are valuable in population genetics research, forensic application, and individual identifications.