应用Cuff技术建立小鼠异位心脏移植模型

A mouse cervical heart transplantation model using a Cuff technique

目的建立简单易行、稳定可靠的小鼠颈部异位心脏移植模型。方法自制血管套管,运用Cuff技术吻合移植心的动、静脉。结果正式实验25次,成功23例,供心缺血时间均少于 30 min。结论该方法明显降低了手术难度,具有手术时间短,创伤小,成功率高的优点,是一种简单实用的方法。

Objective To establish a simple cervical heart transplantation model in mice. Methods Using a Cuff technique the arterial and venous anastomoses were performed in mouse cervical heart transplantation with self-made small Cuffs. Results The graft ischemic time was reduced to less than 30 min and the 3-day survival rate was 92 %. Conclusion This methods has several advantages such as short graft isehemic time, relative small injuries and high success ratio, so it is a simple and useful technique for heart transplantation research.