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淋球菌mtr系统的IR区基因突变与其多重耐药性的关系 被引量:3

The Mutation of IR Gene in the mtr System and Multiple Antibiotic Resistance of Neisseria gonorrhoeae
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摘要 目的探索多传递耐药(mtr)系统反向重复序列(IR)区基因突变与淋球菌染色体介导多重耐药性的关系。方法琼脂稀释法做淋球菌药敏试验,选择耐不同药物的菌株,聚合酶链反应扩增包含 IR区的目的基因,然后对扩增产物测序。结果 2株敏感株及5株仅耐青霉素菌株无IR区基因突变,17 株多重耐药菌株中,1株同时耐青霉素和阿奇霉素的淋球菌有IR区碱基T/A,T/A插入,其余耐药株IR区均有碱基A/T缺失。结论淋球菌染色体mtrR启动子区域的IR区基因突变会引起淋球菌多重耐药株的产生,增加淋球菌对抗生素的抗性。 Objective To study the relationship between the mutation of the reverted repeat (IR) gene in the multiple transferable resistant (mtr) system and multiple antibiotic resistance of Neisseria gonorrhoeae. Methods The antimicrobial susceptibilities of isolated strains were tested. An agar plate dilution method was used to determine the minimum inhibitory concentrations. The target genes were amplified by PCR and subjected to sequencing. Results No mutation was found in the IR gene of either of 2 sensitive or 5 penicillin-resistant Neisseria gonorrhoeae strains. Among the 17 multiple-antibiotic-resistant strains, a strain with both azithromyein- and penicillin-resistance had T/A and T/A insertions, and another had A/T deletion. Conclusion Mutations in the IR gene of the mtr system of Neisseria gonorrhoeae might result in multiple antibiotic resistance.
出处 《中华皮肤科杂志》 CAS CSCD 北大核心 2005年第12期719-721,共3页 Chinese Journal of Dermatology
基金 国家自然科学基金资助项目(30371293)
关键词 奈瑟球菌 淋病 基因 细菌 重复序列 核酸 微生物敏感性试验 Neisseria gonorrhoeae Genes, bacterial Repetitive sequences, nucleic acid Microbial sensitivity tests
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同被引文献18

  • 1陈宏翔,涂亚庭,林能兴,黄长征,张丽霞.淋球菌多重耐药性与mtrR基因点突变的关系[J].华中科技大学学报(医学版),2005,34(5):616-618. 被引量:8
  • 2林能兴,黄长征,连昕,张丽霞,涂亚庭,康建,朱里.淋球菌mtrR调控区突变片段的体外重组和鉴定[J].中国麻风皮肤病杂志,2006,22(2):95-97. 被引量:1
  • 3张丽霞,林能兴,黄长征,陈宏翔,林云,涂亚庭.Relationship between Mutation of IR in the mtr System of Neisseria Gonorrhoeae and Multiple Antibiotic Resistance[J].Journal of Huazhong University of Science and Technology(Medical Sciences),2006,26(2):248-250. 被引量:2
  • 4张丽霞,林能兴,胡志国,黄长征,刘志香,陈宏翔,林云,涂亚庭.不同耐药淋球菌菌株IR基因突变与mtrC基因转录水平的差异性比较[J].临床皮肤科杂志,2006,35(12):763-765. 被引量:1
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