不同氮源形态和植物激素对小球藻USTB01生长及叶黄素含量的效应

Effects of Various Nitrogen Sources and Phytohormones on Growth and Content of Lutein in Chlorella sp USTB01

在光照条件下,分别研究了氯化铵、尿素和硝酸钾3种氮源,以及吲哚乙酸(IAA)和吲哚丁酸(IBA)2种植物激素对小球藻生长及叶黄素含量的效应。结果表明,葡萄糖和硝酸钾分别作为唯一碳源和氮源可以支持小球藻快速持续生长;尿素作为唯一氮源时小球藻生长缓慢,而氯化铵作为唯一氮源时因使培养物中的pH快速降低而抑制了小球藻的进一步生长。与尿素和氯化铵相比,硝酸钾是促进小球藻生物合成叶黄素的最好氮源,小球藻细胞中的叶黄素含量可以达到0.85mg/g。在葡萄糖为碳源和硝酸钾为氮源条件下,加入植物激素IAA、IBA非但不能明显促进小球藻的生长,反而明显抑制了小球藻对叶黄素的生物合成。

The effects of three nitrogen sources such as ammonium chloride, urea and potassium nitrate, and two phytohormones such as indole-3-acetic acid （IAA） and indole-3-butyric acid （IBA）, on the growth and the content of lutein in Chlorella sp were investigated under light condition, respectively. The results showed that when glucose and potassium nitrate were used as the sole carbon and nitrogen sources, the rapid and continuous growth of Chlorella sp was achieved. If urea was used as the sole nitrogen source, the growth of Chlorella sp was distinctly slow. Using ammonium chloride as the sole nitrogen source, the growth of Chlorella sp could be apparently inhibited by the rapid decrease of pH of culture solution. Compared with urea and ammonium chloride, potassium nitrate was the best nitrogen source to promote biosynthesis of lutein by Chlorella sp, and the yield of lutein in dry algal cells could reach 0.85mg/g. In the presence of glucose and potassium nitrate, the growth of Chlorella sp could not be accelerated, on the contrary, the biosynthesis of lutein was inhibited by IAA or IBA, respectively.