菜油油烟凝聚物诱发人羊膜FL细胞中的非定标性突变

NONTARGETED MUTAGENESIS IN HUMAN AMINO FL CELLSINDUCED BY RAPE SEED OIL CONDENSATE

本研究以穿梭质粒ｐＺ１８９作为探针，用菜油油烟凝聚物ＲａｐｅＳｅｅｄＯｉｌＣｏｎｄｅｎｓａｔｅ，ＲＣ）诱发人羊膜ＦＬ细胞中的非定标性突变。结果显示：ｌ２．５和５０μｇ／ｍｌＲＣ处理组的突变频率较自发突变频率增加了３．５和４．１倍；两个处理组的点突变频率分别为１１．９×１Ｏ－４（１９／１５９９６）和１１．６×ｌ０－４（１７／１４７０１），比自发点突变频率２．９×１０－４（２／６８２２）分别增加了４．１和３．９倍，其卡方检验的Ｐ值均小于０．０５。

The nontargeted mutagenesis in human amino FL cells was induced by Rape SeedOiI Condensate(RC)with a shuttle plasmid pZl89 as probe. The results showed that muta-tion frequencies increased by 3.5-and 4.1-fold Over the spontaneous mutation frequency inthe l2.5 and 50μg/ml RC pretreated cells,respectively. The scored point mutation frequen-cies were ll.9×l0-4(19/15996)and ll. 6×10-4(17/14701),in the two dose groups respec-tively. Thus 4.1-and 3.9-fold increased inpoint mutatiOn frequencies were found compar-1ng with the spontaneous mutation frequency of 2. 9×10-4(2/6822).Chi square test showedthat the P value is small than 0.05.