威猛（Vm－26）对Hela细胞染色体形成的影响

THE EFFECTS OF Vm-26 ON THE FORMATION OF HELACELL CHROMOSOME

抗癌药物Ｖｍ－２６是一种拓朴异构酶（ＴｏｐｏｉｓｏｍｅｒａｓｅⅡ，ＴｏｐｏⅡ）及组蛋白Ｈｌ激酶的抑制剂。本文就其对Ｈｅｌａ细胞染色体形成的影响进行了观察。不同浓度的Ｖｍ－２６加入Ｈｅｌａ细胞培养液后４８小时，进行染色体制备，光镜观察。当Ｖｍ－２６的培养浓度在０．５－１．０μｇ／ｍｌ时，染色体的形成被阻断于分裂前期。当药物培养浓度于０．２５μｇ／ｍｌ时，可见有相当数量的细胞处于分裂前期，其胞核内具有不规则的染色质凝缩。另外，尚可见极少的分裂中期染色体集簇形成，但大都伴有染色体的结构异常。结果提示：Ｖｍ－２６可以通过抑制Ｈｅｌａ细胞染色体的形成，而影响细胞正常的分裂、繁殖。

The antineoplastic agent,teniposide(Vm-26)is a inhibitor of the topoisomeraseⅡ(TopoⅡ).It can block the histone Hl phoshorylation. Its effects on the formation of Helacell chromosome was studied. The cell were cultured in diHerent concentration of Vm-26for 48 hours.Through preparation and observation of the chromosome with the conventionalrnethod,we noted that the chromosomal formation was blocked at G2 phase when the Vm- 26 was in a cuItured concentration of 0.5-1.0μg/ml.Mitotic cells were found to be pres- ence in 0.25μg/ml Vm- 26,but most of them failed to condense into the normal metaphasespreads,which always showed an irregular chromatin substance in the nuclei,or structure al- tered chromosomes.The results indicated that Vm- 26 can blocked Hela cell division by in-hibiting its chromosome formation,mainly acting on the late step of chromosome condensa- tion,while the chromatin fibers condense to form mitotic chromosome.