动脉粥样硬化与VCAM-1及CD36关系的实验研究

Study of relation between VCAM-1,CD36 and atherosclerosis in rabbits

目的:探讨动脉粥样硬化(AS)时血脂成分与VCAM-1及CD36蛋白在兔AS发生中的作用及其机制。方法:采用HE、免疫组化染色,光镜观察AS病变,并应用CMIAS真彩色医学图像分析系统,检测40只食饵性AS新西兰兔,15周处死,观察其血脂成分及血管壁的病理变化、VCAM-1及CD36蛋白的表达情况。结果:实验兔最终血TC升高约43倍,最终血LDL升高约37倍。主动脉内壁可见脂质条纹形成。检测主动脉AS病变VCAM-1、CD36蛋白表达定量A值,分别为:0.19±0.02、0.20±0.04,显著高于对照组:0.07±0.01、0.03±0.02的定量表达(P<0.05)。结论:明确了高血脂症是AS发病的基础,提示VCAM-1及CD36蛋白表达增多可能是致AS的机制之一。

Objective： To study the role of serum lipid （TC, TG, LDL-C） VCAM-1,CD36 and the mechanisms in the occurrence and process of atherosclerosis. Methods： Blood vessel wall, the serum lipid and the expressions of VCAM-1,CD36 were observed in dietary induced atherosclerosis in 40 rabbits models by morphology study, immunohistochemistry, insitu hybridization technique and color image analyzer （CMAIS）. Results： The models of AS were established successfully after rabbits were fed with cholesterol, which developed the fatty streak lesion in the aortic intra-wall. In the rabbit models, TC and LDL were increased 43 times respectively and 37 times VCAM-1 and CD36 were 0.19±0.02 and 0.20±0. 04, which were higher than control group 0. 07 ± 0. 01 and 0. 03 ± 0. 02 respectively （P〈0. 05）. Conclusion.. The resule suggests that hyperlipiderhia is one of the uppermost causes of AS ,and that VCAM-1 and CD36 is one of the mechanisms causing AS.