键合偶联双奎宁手性固定相的制备和手性拆分性能

Preparation of a Bis-Quinine Bonded Chiral Stationary Phase and Its Chiral Resolution Ability

A novel chiral stationary phase(CSP) was prepared by immobilizing coupled quinine,which was synthesized by the reaction of quinine with 4,4′-diphenyl methane diisocyanate(MDI),onto the surface of(3-mercaptopropyl-)silanized silica gel via radical reaction in the presence of 2,2′-azobisisobutyronitrile(AIBN).With the conventional slurry packing method,a column was packed with the newly synthesized CSP material.By means of high performance liquid chromatography,the chromatographic properties of this column were tested with five chiral compounds under different mobile phases.The results obtained indicate that(chloropheniramine),1,1′-bi-2-naphthol,promethazine and propranolol were resolved into their enantiomers,and their separation factors were 2.08,1.27,1.43 and 1.16,respectively.Furthermore,a possible chiral(resolution) mechanism was proposed.

A novel chiral stationary phase （CSP） was prepared by immobilizing coupled quinine, which was synthesized by the reaction of quinine with 4,4＇-diphenyl methane diisocyanate（MDI）, onto the surface of 3-mercaptopropyl-silanized silica gel via radical reaction in the presence of 2, 2＇-azobisisobutyronitrile （AIBN）. With the conventional slurry packing method, a column was packed with the newly synthesized CSP material. By means of high performance liquid chromatography, the chromatographic properties of this column were tested with five chiral compounds under different mobile phases. The results obtained indicate that chlornpheniramine, 1,1 ＇-bi-2-naphthol, promethazine and propranoM were resolved into their enantiomers, and their separation factors were 2. 08, 1.27, 1.43 and 1.16, respectively. Furthermore, a possible chiral resolution mechanism was proposed.