培养过程参数对霉菌Rhizopus oryzae IFO细胞催化植物油脂合成生物柴油的影响研究

Effect of Cultivation parameters on Rhizopus oryzae IFO Cell-catalyzed Transesterification of Vegetable Oils for Biodiesel Production

采用脂肪酶催化可再生动植物油脂合成生物柴油已经成为目前研究的热点,其中利用全细胞催化剂是一个重要的研究方向。文中直接利用霉菌R.oryzaeIFO细胞催化植物油脂甲醇解反应合成生物柴油,系统研究了培养过程参数对细胞生长和该细胞催化剂催化甲醇解反应活性的影响。研究表明,细胞培养过程中所添加的油脂不同,细胞在后续反应中催化特定油脂进行生物柴油制备时所表现出的催化活性也会有所差别;由某种油脂作为碳源得到的细胞催化剂催化对应油脂与甲醇转酯化反应制备生物柴油时,表现出比催化其他油脂和甲醇反应制备生物柴油更高的催化活性。在优化的操作参数(大豆精制油20g/L,蛋白胨70g/L,NaNO31.2g/L,KH2PO41.2g/L,MgSO4·7H2O0.5g/L,培养温度35℃,摇床转速130r/min)下,培养得到的细胞催化剂能有效催化大豆油与甲醇三步转酯化反应,生物柴油(脂肪酸甲酯)最终得率可达到86%。

Enzymatic transesterification for biodiesel production has become a hot research field recently, in which study on whole cell as catalyst as an important lead. In this paper, whole cell （R. oryzae IFO 4697） was adopted directly as biocatalyst. Effects of different cultivation parameters on cell growth and whole cell-catalyzed transesterification of vegetable oils for biodiesel production were studied. The results showed that different oils contained in the cultivation medium had varied effects on the whole cell-catalyzed transesterification of vegetable oils for biodiesel production. With some specific oil as the Carbon source for cell cultivation, those ceils would express higher catalytic activity in catalyzing the transesterification of the same oil for biodiesel production. Under the optimized conditions（ refined soybean oil 20 g/L, peptone 70 g/L, KH2PO4 1.2 g/L, MgSO4· 7H2O 0.5 g/L, NaNO3 1.2 g/L, 35℃ and 130 r/min）, the maximum methyl ester yield could reach 86%.