响应面法优化莲藕淀粉的酶解工艺参数研究

Optimizing Amylase Usage in Pretreatment of Lotus Root with Response Surface Methodology

研究了淀粉酶反应温度和作用时间及酶用量对莲藕浆液酶解效果的影响,在单因素试验基础上,采用通用旋转设计和响应面分析莲藕浆液淀粉酶酶解的最优条件。结果表明,莲藕淀粉的最佳酶解工艺参数为:淀粉酶用量0.15%,反应温度60.69℃,作用时间116.35min。

The enzymolytical effect of temperature,time and amylase addition on lotus root pulp was studied. The optimum process parameters were determined with a second-order central composite rotatable design in combination with response surface methodology. The optimum combination was 0.15% amylase addition, 60.69℃, 116.35 min.