吐温80增溶-紫外分光光度法测定辅酶Q_(10)脂质体的载量及包封率

Determination of Loading Capacity and Encapsulation Efficiency of Coenzyme Q_(10) Liposomes by Tween 80 Solubilization and UV Spectrophotometry

在辅酶Q10脂质体的制备过程中,载量和包封率是评价辅酶Q10脂质体的2个重要质量指标。采用表面活性剂吐温80对辅酶Q10脂质体进行增溶,再结合紫外分光光度法测定其载量和包封率。研究结果表明,辅酶Q10浓度在2.5～50μg/mL范围内,吐温80增溶法与以乙醇为溶剂的反相高效液相色谱法以及紫外分光光度法有良好的相关性(R2>0.999);空白脂质体中,辅酶Q10的加样回收率在(98.26±0.63)%～(101.20±1.28)%之间,相对标准偏差RSD<2%(n=6);该法用于测定辅酶Q10脂质体中总辅酶Q10含量的RSD<5%(n=6);不同载量[(3.22±0.01)%～(13.62±0.31)%]的辅酶Q10脂质体的包封率均高于95%(RSD<1%,n=6)。与以乙醇为溶剂的反相高效液相色谱法以及紫外分光光度法相比,该法具有准确可靠、简单、重现性较好的优点。

The loading capacity and encapsulation efficiency are important indexes to evaluate the quality of coenzyme Q10 liposomes. The two indexes of coenzyme Q10 liposomes were analyzed by surfactant Tween 80 solubi- lization and ultraviolet （UV） spectrophotometry in this study, This method had a good correlation with ethanol solubilization and RP-HPLC or UV spectrometry in the range of 2.5 -- 50 μg/mL of coenzyme Q10 （R^2 〉0. 999）, The recoveries of coenzyme Q10 in blank liposomes ranged from （98.26 ± 0.63） % to （101.20 ± 1.28） % with relative standard deviation（RSD） less than 2 %. When the proposed method was applied to determine the loading capacity and encapsulation efficiency of coenzyme Q10 liposomes, the RSD were less than 5 % and 1% respectively, and the encapsulation efficiency were all higher than 95 % with loading capacity ranging from（3.22 ± 0.01）% to （13.62 ± 0.31）%. The method was proved to be convenient, sensitive, accurate and reproducible compared with conventional RP-HPLC and UV spectrophotometry with ethanol solubilization.