骨修复材料多孔聚磷酸钙的制备及细胞相容性研究

Synthesis and In-vitro Cell Compatibility Study of Porous Calcium Polyphosphate

目的为了解决骨组织工程中成骨细胞在可降解支架材料上三维生长这一问题,本文对无机聚合物聚磷酸钙(CPP)生物陶瓷进行了研究。方法实验制备出CPP多孔材料,并以羟基磷灰石,磷酸三钙作为对照材料,检测了材料的细胞毒性,并与成骨细胞进行复合生长。结果表明聚磷酸钙不仅无细胞毒性,而且能促进成骨细胞的生长,与成骨细胞复合后,细胞能在材料中三维生长,并能分泌出细胞外基质,因此具有良好的细胞相容性。结论多孔聚磷酸钙能促进成骨细胞生长,并为细胞良好生长提供三维空间环境,同时本实验也为可降解无机聚合物作为骨组织工程支架材料的研究提供了新的思路。

Objective To help in solving the problem of growth of osteoblasts on biodegradable scaffolds in bone tissue engineering. Method Porous calcium polyphosphate （CPP） scaffolds were prepared and its cell toxicity was compared with those of hydroxyapatite and tricalcium phosphate. Osteoblasts were made into composite with CPP and grew on it to judge the osteoconduction. Result Results showed that CPP had good cell compatibility and could promote growth of the osteoblasts. The osteoblasts could grow three-dimensionally on porous CPP and excrete extracellular matrix. Conclusion Porous CPP can promote the growth of osteoblasts and provide a three-dimensional scaffold for cell growth. It provides a new research aspect of biodegradable inorganic polymer as scaffolds for bone tissue engineering.