模拟失重对胃粘膜上皮细胞增殖和周期的影响

Effects of Simulated Weightlessness on Cellular Morphology and Cell Cycle of Cell Line HFE-145

目的探讨模拟失重对胃粘膜上皮HFE-145细胞增殖和周期的影响。方法采用回转器模拟失重,用免疫组化法观察HFE-145细胞PCNA表达的变化,用流式细胞仪测定细胞周期。结果回转器模拟失重后,与正常对照组相比,回转组HFE-145细胞24、36、48及72h的PCNA抗体阳性细胞比率无明显差异,12h回转组阳性细胞比率明显减少(P<0.05)。24、36、48、72h回转组HFE-145细胞的细胞周期分布与对照组无明显差异,12h回转组G0+G1期细胞比例明显增多(P<0.05),S+G2+M期细胞之和显著减少(P<0.05)。结论回转器模拟失重使胃粘膜上皮HFE-145细胞在12h时出现了细胞周期阻滞和增殖抑制。

Objective To investigate the effects of simulated weightlessness on proliferation and cell cycle of human gastric mucosa cell line HFE-145, Method A rotating clinostat was used to simulated weightlessness. The expressions of proliferating cell nuclear antigen（PCNA） were examined by immunohistochemical stain. The changes of cell cycle were examined by a cytometer, Result Compared with control, the expression of PCNA of cell line HFE-145 showed no significant change after 24 h, 36 h, 48 h and 72 h simulated weightlessness, but decreased significantly after 12 h simulated weightlessness （P 〈0.05）, As compared with control, the cell cycles of cell line HFE-145 in 24 h, 36 h, 48 h and 72 h groups showed no significant change, but the cells in G0 ＋ G1 phase after 12 h simulated weightlessness increased significantly（P 〈0.05）, and the cells in S ＋ G2 ＋ M phase decreased significantly（P 〈0. 05）, Conclusion The proliferation of HFE-145 cell is inhibited during simulated weightlessness on a clinostat for 12 h. The conversion of cell from G1 phase to S phase is also blocked by 12 h simulated weightlessness.