铝螯合剂对尿中微量元素排出的影响

Effect of aluminum chelating agent on urinary trace elements excretion in rabbits

目的探讨不同剂量铝螯合剂1,2-二甲基-3-羟基-4-吡啶酮(DHPO)在铝中毒排铝时,对其他微量元素铜、锌、锰在尿中排出的影响。方法雄性新西兰大白兔24只,随机分为对照组、低、中、高剂量组。在2周内每天皮下注射染铝600μmol/kg,每周5 d。将DHPO的灭菌水溶液分别按500,750,1 000μmol/kg给低、中、高剂量组灌胃,收集给螯合剂前1 h和后6 h的尿液,用原子吸收分光光度法测微量元素含量。结果给DHPO后,低、中、高剂量组分别与对照组比较尿铝排出明显增加(P<0.001);铜、锌、锰排出量与对照组相比差异均无统计学意义(P>0.05)。结论DHPO对尿中铝的排出明显,而对铜、锌、锰在尿中的排出无影响。

Objective To reveal the influence of 1, 2 - dimethyl - 3 - hydroxypyrid - 4 - one （DHPO） on the urinary trace elements, Copper（Cu）, Zinc（Zn）and Manganese（Mn） excretion while it eliminatd aluminum in Al- loaded rabbits. Methods 24 male Newzealand white rabbits were randomly divided into 4 groups： control, low dose group, middle dose group and high dose group, The animals received aluminum dose [ （600 μmol/（ kg· d）, 5 d/w） ]for two weeks by subcutaneous injection, and after the last injection, the DHPO sterile solution of 500,750, 1000 ymol/kg dose were given to low dose group, middle dose group and high dose group intragastrically, The urine were collected and the trace dements contents were analyzed by AAS.Results DHPO could highly increase aluminum output, whereas the excretion of urinary Cu, Zn, Mn contents in the groups administered with DHPO was not significantly different comparing with that in control group. Conclusion DHPO may effectively enhance the elimination of aluminum and exert little effect on urinary Cu, Zn, Mn excretion.