杜氏盐藻中的核基质与核基质结合区(英文)

Nuclear Matrices and Matrix Attachment Regions from Green Alga:Dunaliella salina

真核生物细胞核DNA通过核基质结合区(Matrixattachmentregion,MAR)附着到核基质上。为了进一步探索染色体DNA与核基质之间的相互作用,从单细胞真核藻类杜氏盐藻中克隆出了MAR片段。首先构建了杜氏盐藻的随机MAR文库,通过体外结合实验分离出能与核基质结合的MAR序列。从构建的MAR文库中,筛选出3个能与核基质结合的MAR,其中两个片段与核基质具有较强的结合力,测序分析表明具有MAR片段的一些典型特征性基序。

Nuclear DNA of eukaryotic organism attaches to the proteinaceous nuclear matrices via specific matrix attachment regions （MARs）. In order to investigate the interactions between chromosomal DNA and nuclear matrices,we isolated the MARs from unicellular alga Dunaliella salina. As the first step, a random MAR library was set up and then the binding affinity of the selected clones to nuclear matrices was tested in this study. Three DNA fragments were found to bind specifically to the nuclear matrices in vitro,of which two were strong binders and all contained known consensus motifs and a hairpin loop structure of MAR.