鲜法匀浆萃取烟叶中茄尼醇的研究

Homogenate Extraction of Solanesol from Fresh Tobacco Leaves

为了建立一种高效提取烟叶中茄尼醇的方法,对鲜法匀浆萃取烟叶中茄尼醇的工艺进行了研究,确定了最佳的工艺参数:提取原料为含水率80%的鲜烟叶,溶剂为90%的乙醇,匀浆时间20min,液固比为6:1(mL:g).将该法与超声提取、索氏提取和超临界二氧化碳萃取进行了比较.结果表明,在优化的条件下,鲜法匀浆对原料的匀浆粒度为40～80μm,茄尼醇的提取率为0.722%,分别是超声提取、索氏提取、超临界二氧化碳萃取的1.32、1.62和1.98倍.鲜法匀浆萃取所用提取时间短,提取溶剂用量少,因此,该法是一种高效提取烟叶中茄尼醇的方法.

In order to find a method with higher efficiency to extract the solanesol from fresh tobacco leaves, the homogenate extraction was studied and the comparisons of the studied method with other extractive methods such as ultrasonic, Soxhlet and supercriticle CO2 extractions were conducted. For the homoganate extraction of solanesol from the fresh tobacco leaves（coutaining 80%water）, the optimum technology parameters were found as following ： homogenate for 20 min with 90% ethanol at a ratio of liquid to solid of 6： 1（mL：g）. Using the above optimized parameters, the granularity of the homogenated material is 40-80μm and the extraction yield of solanesol is 0.722% which is 1.32, 1.62 and 1.98 times higher than the yield using ultrasonic, Soxhlet and supercritide CO2 extracting methods, respectively. Furthermore, the proposed homogenate extraction method needs less extractive time and less extraction solvent, so it is a highly efficient method for extracting solanesol from fresh tobacco leaves.