浙南汉族人群18号染色体上三个STR位点的遗传多态性研究

Genetic polymorphism of 3 STR loci on chromosome 18 in Wenzhou Han population.

目的 研究浙南汉族人群18号染色体上三个STR位点D18S865、D18S1364和D18S535的遗传多态性分布,获得相 应的群体遗传学数据。方法 应用聚合酶链式反应(PCR)、聚丙烯酰胺凝胶电泳(PAGE)及银染显带技术对浙南地区汉族184 名无关个体的D18S865、D18S1364和D18S535位点进行分型。结果 D18S865观察到6个等位基因及17种基因型,D18S1364观 察到9个等位基因及35种基因型,D18s535观察到8个等位基因及29种基因型,多态性分布都符合Hardy-Weinberg平衡。各 基因座的杂合度(H)分别为:0．723、0．848、0．783;多态性信息含量(PIC):0．72、0．81、0．79。结论 三个STR基因座具有较高杂 合度和多态性信息含量,在遗传学研究和法医学应用中有较高的价值。

Objective： To investigate genetic polymorphism of D18S865, D18S1364 and D18S535 in South Zhejiang Han population. Methods： Population studies on D18S865,D18S1364 and D18S535 were carried out in a sample of 184 unrelated South Zhejiang Han individuals using PCR followed by PAGE and silver stain detection. Results ： 6,9 , 8 alleles and 17,35,29 genotypes of D18S865, D18S1364 , D18S535 were respectively observed among the 184 unrelated individuals. The genotype frequency match the Hardy- Weinberg equilibrium, The heterozygotes（H） in D18S865, D18S1364 and D18S535 were 0.723,0. 848,0. 783, polymorphism information content（PIC） were 0.72,0.81,0.79 respectively. Conclusion： D18S865,D18S1364 and D18S535 had high heterozygotes and polymorphism information content, so the three loci can be used in genetic researches and in forensic medicine.