原发性高血压与ACE基因的多态性关联在蒙古族人群的研究

The polymorphism in intron 16 of ACE in the populations of Chinese Mongolians in essential hyperension

目的 调查内蒙古地区蒙古族原发性高血压人群的ACE基因I/N的多态性,并同蒙古族健康人群比较,分析其 遗传特点。方法 应用ACE基因内含子16多态部位侧翼的前体进行PCR扩增。等位基因在琼脂胶上电泳。溴乙啶染色,紫 外灯下观察。结果 蒙古族高血压人群的DD,DI和II基因型的频率是0．47,0．32和0．21。蒙古族健康人群的DD,DI和II 基因型的频率是0．51,0 26和0．23。结论 在蒙古族人群的研究中未发现ACE基因I/N的多态性与原发性高血压相关。

Objective： To investigate the Insertion/Deletion （I/N） polymorphlsm of angiotensin converting enzyme （ACE） in essential hypertension in Mongolians and analysis their genetics characteristics. Methods： Genomic DNA was amplified by polymorphic region in intron 16 of the ACE gene. Allele were detected on agarose gel stained with ethidium bromide. Results： The frequencies of DD, DI and Ⅱ genotype were 0.51, 0.26 and 0.23 in control; and 0.47, 0.32 and 0.21 in the essential hypertension respectively. Conclusion ： The polymorphism of ACE gene are not differences in essential hypertension in Mongolian.