怀化地区侗族高血压高发人群醛固酮合成酶基因多态性(-344C/T)研究

Polymorphism analysis of- 344C/T of the CYB11B2 gene in high risk population of hypertension of Dong Nationality group in Huaihua district or Hunan

目的探讨醛固酮合成酶(CYB11B2)基因多态性与怀化侗族高血压高发人群原发性高血压(EH)及血脂水平的关 系 方法 采用聚合酶链反应结合限制性内切酶片段长度多态分析方法(PCR-RFLP)检测89例怀化侗族高血压病人和85 例正常人的CYB11B2-344C/T等位基因频率和基因型频率。结果 高血压组CYB11B2-344C/T基因型频率(CC 10.1% CT 41．6％、TT 48．3％)和等位基因频率(C 30．9％、T 69．1％)与正常对照组基因型频率(CC 10．3％、CT 27．6％、TT 62.1％)和等位 基因频率(C 24．1％、T 75．9％)比较无显著性差异。CC基因型患者与(CT+TT基因型患者比较,收缩压和舒张压无显著性差 异。结论 怀化侗族人群FH可能与CYB11R2基因多态性无关。

Objective：To investigate the relationship between CYB11B2 gene polymorphism and esseaatial hypertension（EH） in the high risk population or Dong nationality group in Huaihua distict of Hunan Province. Methods：PCR- RFLP was used for genotyping of CYB11B2 - 344C/T in 89 patients with EH and 85 healthy control subjects. Results：There were no significant differences of the CYB11B2 polymorphism genotypes and alleles between 89 hypertensive patients（CC10.1% ,CT41.6% ,TT48.3% ;C30.9%, T69.1% ）and 85 healthy control subjects （ CC10.3%, CT27.6%, TT2.1% ）, The systolic blood pressure （SBP） and diastolic blood pressure （DBP）did not differ significantly between CC and CT ＋TT genotypes in hypertensive patients. Conclusion：The - 344C/T polymorphism of the CYB11B2 gene was not associated with EH in the population of Dong nationality group in Huaihua district.