卡马西平诱导大鼠骨髓抑制模型的建立

Establishment of rat bone marrow inhibition model induced by carbamazepine

目的:建立卡马西平(CBZ)诱导的骨髓抑制大鼠模型。方法:SD大鼠72只,随机分为正常对照组和模型组。正常对照组12只,每d灌胃蒸馏水,其余不做任何处理。模型组60只,以CBZ灌胃,剂量由600mg/kg始,每5d增加200mg/kg,至1200mg/kg维持,共计灌胃120d,然后停用CBZ,继续观察30d。于给药第30d、60d、90d、120d及停药30d后各取大鼠12只处死,观察外周血象、骨髓象的变化。结果:模型组大鼠一般情况差,给药60d后大鼠即开始表现全血抑制,其中红系造血母细胞的抑制更为明显。在CBZ撤除30d内,造血系统功能未见明显恢复。结论:成功建立了CBZ诱导的骨髓抑制大鼠模型。

Aim ： To establish the model of rat bone marrow inhibition induced by carbamazepine tablets （ CBZ）. Methods： A total of 72 rats were randomly allocated into 2 groups. The model group（ n = 60） was given CBZ by gavage from a dose of 600 mg/kg and increased by 200 mg/kg every 5 d till increased to 1 200 mg/kg and mainained the dose till the end of administration. The normal control group（ n = 12） was given distilled water. On the 30th d,60th d,90th d, 120th d,and 150th d,12 rats in model group and rats in normal control group on the 150th d were executed. The changes of peripheral hemogram and bone marrow matrix were observed. Results ：The general condition of model group was bad and from the 60th d,the rats presented the whole blood inhibition, and the inhibition of hemocytoblast was more obvious. Within 30 d after the withdrawl of CBZ, the bone marrow function had no obvious recovery. Conclusion ： The rat bone marrow inhibition model induced by CBZ is successfully established.