复康片、肌苷和吗丁啉对大鼠卡马西平血药浓度的影响

Effect of Fukang tablet,inosine and motilium on blood carbamazepine concentration in rats

目的:观察复康片、肌苷和吗丁啉对大鼠卡马西平(CBZ)血药浓度的影响。方法:SD大鼠随机分为6组,每组16只。正常对照组每d灌胃蒸馏水,其余不做任何处理;余5组以分步逐渐增加灌胃剂量法灌服CBZ90d。复康片低剂量组、高剂量组、肌苷组和吗丁啉组灌服CBZ同时分别灌胃复康片750mg/(kg·d)、1500mg/(kg·d)、肌苷600mg/(kg·d)和吗丁啉15mg/(kg·d),每d1次。各组于灌服CBZ第60d、90d、120d及CBZ停药30d后(150d)眼眶静脉丛采血,用高效液相色谱法检测CBZ血药浓度。结果:正常对照组血清中未检出CBZ。不同时间点复康片组、肌苷组、吗丁啉组及模型组大鼠CBZ血药浓度组间比较,差异均无统计学意义。结论:复康片、肌苷和吗丁啉灌胃对大鼠CBZ血药浓度无明显影响。

Aim： To observe the effect of Fukang tablets, inosine, and motilium on blood carbamazepine（CBZ） concentration in rats. Methods： A total of 96 rats were randomly allocated into 6 groups：normal control group was given distilled water, and the other 5 groups were given CBZ for 90 d,among which,model group was only given CBZ,and low-dose Fukang tablet group,high-dose Fukang tablet group,inosine group,and motilium group were given 750 mg/kg Fukang tablet, 1 500 mg/kg Fukang tablet,60 mg/kg inosine,and 15 mg/kg motilium per day by gavage. On the 60th d,90th d, 120th d,and 150th d,blood samples were prepared for determining the blood CBZ concentration using HPLC. Results： CBZ could not be detected in the normal control group. There were no significant differences in the blood CBZ concentration among other 5 groups at each time point. Conclusion： Fukang tablet,inosine,and motilium have no effects on the blood CBZ concentration in rats.