川芎嗪对抗原诱发致敏豚鼠气管平滑肌环的舒张作用

Effect of ligustrazine on tracheal smooth muscle isolated from guinea pigs with induced asthma

目的:观察川芎嗪对过敏原诱发的哮喘豚鼠离体气管平滑肌环收缩的影响及其相关机制。方法:采用卵蛋白致敏法建立豚鼠哮喘动物模型,每只豚鼠制备5～7个气管平滑肌环,悬挂于恒温浴槽中,并将其与力位移换能器相连来纪录气管环张力的变化,所有标本分为正常KH液和无钙KH液2大组。正常KH液组又分为卵蛋白组、10mmol/L川芎嗪+卵蛋白组、30mmol/L川芎嗪+卵蛋白组、100mmol/L川芎嗪+卵蛋白组、300mmol/L川芎嗪+卵蛋白组、thapsigargin(TSG)+卵蛋白组、TSG+川芎嗪(100mmol/L)+卵蛋白组。无钙KH液组分为卵蛋白组和川芎嗪(100mmol/L)+卵蛋白组。结果:①卵蛋白组平滑肌环收缩产生的张力为(59.7±8.5)×10-6N,分别预先加入30mmol/L、100mmol/L、300mmol/L的川芎嗪20min后,再加入卵蛋白,平滑肌环产生的张力分别降低至(44.1±5.5)×10-6N、(20.7±20.4)×10-6N、(10.0±1.1)×10-6N(P均<0.05),10mmol/L的川芎嗪对卵蛋白的作用无明显影响(P>0.05);②去除溶液中的Ca2+后,再加入卵蛋白,平滑肌环收缩产生的张力为(10.5±1.9)×10-6N,预先加入100mmol/L的川芎嗪后,卵蛋白诱导产生的平滑肌环的收缩力无明显改变(P>0.05);③预先加入TSG(10mmol/L)40min后,再加入卵蛋白,平滑肌环收缩产生的张力为(27.3±3.4)×10-6N,分别预先加入TSG和100mmol/L的川芎嗪后,再加入卵蛋白诱导,平滑肌环的收缩力减少至(6.5±1.1)×10-6N(P<0.05)。结论:①川芎嗪可能通过抑制细胞膜上的钙通道来抑制抗原引起的哮喘豚鼠离体气管平滑肌的收缩作用。②抗原可能通过细胞外的Ca2+内流和细胞内肌浆网对Ca2+的释放来促进哮喘豚鼠离体气管平滑肌的收缩。

Aim ： To investigate the effect of ligustrazine on tracheal smooth muscle isolated from guinea pigs with induced asthma and the relative mechanism. Methods： Asthma animal model was established using ovalbumin,and tracheal smooth muscle（TSM） rings were prepared and connected to force-displacement transducer so that the contraction of TSM could be recorded. The roles of extracellular and intracellular Ca^2＋ in the effect of liguatrazine were studied. Results ：Ligustrazine inhibited the contraction of TSM induced by antigen（ovalbumin） in a dose dependent manner. In Ca^2＋-free solution, ligustrazine could not inhibit the contraction of TSM induced by antigen. After addition of thapsigargin for 40 min, ligustrazine inhibited the contraction of TSM induced by antigen significantly. Conclusion： Ligustrazine could inhibit the ovalbumin-induced contraction of TSM by blocking Ca^2＋ channel on the membrane. Extracellular and intracellular Ca^2＋ play a role in the contraction of TSM induced by antigen.