乳癌组织中PTEN的表达

Expression of PTEN in breast carcinoma tissue

目的:探讨乳癌组织中第10号染色体缺失的磷酸酶和张力蛋白同源物基因(PTEN)的表达及其意义。方法:采用免疫组织化学SP方法,检测74例乳癌组织(TNM分期:Ⅰ期18例,Ⅱ期31例,Ⅲ期25例。有腋窝淋巴结转移40例,无转移者34例)和20例正常乳腺组织中PTEN蛋白的表达水平。另取20例正常乳腺组织为对照。结果:乳癌组织中PTEN蛋白的阳性率和阳性强度均低于正常乳腺组织(P<0.01);腋淋巴结转移组的乳癌组织中PTEN蛋白阳性率明显低于无淋巴结转移组(P<0.05);随着临床分期的增高,PTEN蛋白阳性表达率呈下降趋势(P<0.05);PTEN表达水平与患者年龄、肿瘤大小无关。结论:PTEN蛋白的异常表达与乳癌的发生发展密切相关,通过检测PTEN蛋白的表达,对估计乳癌的预后有一定的意义。

Aim： To investigate the expression of PTEN and its clinical significance in breast carcinoma tissue. Methods： Expression of PTEN in 74 cases of breast carcinoma and 20 normal breast tissue was detected using immunohistochemistry SP method. Results： The positive rate and expressive intensity of PTEN protein in breast carcinoma tissue were lower than those in normal breast tissue （ P 〈 0.01 ） ; the positive rate of PTEN in axillary lymph node metastasis group was lower than that in the group without axillary lymph node metastasis （ P 〈 0.05 ） ; the positive rate of PTEN was correlated with clinical stage （ P 〈 0.05 ） , and with the increasing of clinical stage, the positive rate of PTEN was declined, but it was not correlated with age and tumor size. Conclusion ： Abnormal expression of PTEN is closely associated with the development of breast carcinoma. It is helpful to assess the prognosis of breast carcinoma by detecting the expression of PTEN.