成人急性髓细胞系白血病免疫分型特点

Immunophenotyping characteristics of 156 adult patients with acute myeloid leukemia

目的:探讨成人急性髓细胞系白血病(AML)三色流式细胞术免疫分型特点及AML抗原表达规律。方法:采用CD45/侧散射(SSC)双参数散点图设门方法对156例成人AML进行三色流式细胞术免疫表型分析。结果:156例AML主要表达CD117(90.4%)、CD13(87.2%)、CD33(71.8%)、CD34(64.7%)、HLADR(60.9%)。34.0%的AML患者伴有淋系抗原表达,最常见为CD7(24.4%)。结论:流式细胞仪三色荧光标记法进行AML免疫分型,对白血病的准确分型诊断、发生发展和预后判断有重要的指导意义。

Aim ： To evaluate the specificity of three-color flow cytometry used in the immunophenotyping in adult patients with acute myeloid leukemia（AML）. Methods： Immunophenotyping was performed by three-color flow cytometry using CD45/SSC gating. R6sults： In 156 patients with AML, the positively expressed antigens were mainly CD117 （90.4%）,CD13（87.2%） ,CD33（71.8%）,CD34（64.7%）,and HLA-DR（60.9%）. Lymphoid-associated antigens expressed in 34.0% of the AML patients,among which,CD7 expressed in 24.4% of the AML patients. Conclusion： Threecolor flow cytometry is important during diagnosis, and judging the development and prognosis of AML.