一株毒死蜱降解细菌的分离鉴定及其在土壤修复中的应用

Isolation and characterization of a chlorpyrifos degrading bacteria and its bioremediation application in the soil

从蔬菜大棚土壤中分离到一株能以毒死蜱为唯一碳源和能源生长的菌株DSP3,该菌在含毒死蜱(100mgL)的酵母膏和蛋白胨与同样毒死蜱含量而无酵母膏蛋白胨的无机盐培养基中,18d对毒死蜱的降解率分别为98.6%和76.2%;在土壤实验中20d对毒死蜱(100mgkg)的降解率接近100%,加入DSP3菌在蔬菜大棚新鲜土壤中能有效促进毒死蜱在土壤中的降解。根据生理生化特征、16SrDNA序列分析、(G+C)mol%测定和DNA同源性分析,将菌株DSP3鉴定为粪产碱杆菌(Alcaligenes faecalis)。

A strain DSP3 capable of utilizing chlorpyrifos as the sole carbon and energy sources was isolated. Based on the results of phenotypic features,phylogenetic of 16S rDNA sequence,DNA （G ＋ C） mol% and DNA homology between strain DSP3 and reference strains, the strain DSP3 is identified as Alcaligenes faecalis. The degradation rate of chlorpyrifos was at 98.6% （100mg/L） in liquid culture medium within 18 days and nearly 100% （100mg/kg） in soil within 20 days respectively. An addition of strain DSP3 （10s cells/g） to soil resuhed in a higher d noninoculated soils. The different degrading rate of chlorpyrifos in four types of treated soils suggestsegradation rate than that the dissipation is mediated by the activity of the soil microorganisms.